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Détails du produit
- ItemsSize 2mgSize 10mg (5mg × 2)Particle size2 μm2 μmPhysical appearancePowder mixturePowder mixtureSourceGln 31 - Thr 274 (Accession # P09758-1)Gln 31 - Thr 274 (Accession # P09758-1)Amount of Coupled Protein＞398 pmol (20 μg) TROP-2/mg beads＞398 pmol (20 μg) TROP-2/mg beadsFormulationPBS, pH7.4, with 10% TrehalosePBS, pH7.4, with 10% TrehaloseReconstitution2 mL sterile deionized water (1 mg beads/mL)5 mL sterile deionized water (1 mg beads/mL)
Product Description
The Human TROP-2-coupled Magnetic Beads is produced by coupling biotinylated TROP-2 heterodimer to streptavidin-conjugated magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the biotinylated protein can bind to the SA beads irreversibly. The pre-coupled beads are ready to use for capturing anti-TROP-2 antibody from your sample with high specificity.
Application
The beads is intended for immunocapture, biopanning. This is a non-sterile product.
* The Isotype control (Cat. No. SMB-B01) is sold separately.
Reconstitution
See Certificate of Analysis (CoA) for detailed instruction.
Storage
This product is stable in storage under the following conditions: -20℃ for 24 months in lyophilized state.
Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
Assay Principles
Antibody Purification:
1. Resuspend the lyophilized beads by adding the buffer of choice.
2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein.
3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads.
4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.
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Typical Data
Immobilized over 20 μg TROP-2 on 1 mg beads can bind Anti-TROP-2 mAb with an EC50 of 0.6924 μg/mL (QC tested).
Protocol
Accelerated stability test. After placing the lyophilized beads at 37°C for 7 days, binding activity between the Human TROP-2-coupled Magnetic Beads (Cat.No. MBS-K028) and anti-trop-2 mAb showed little deviation from the unaccelerated sample (%RSD<10%). Data were measured on day 0, 3, 7 respectively.
Protocol
Freeze-thaw stability test. After different freeze-thaw cycles, binding activity between the Human TROP-2-coupled Magnetic Beads (Cat.No. MBS-K028) and anti-trop-2 mAb showed little deviation from the unfreeze-thaw sample (%RSD<10%). Three freeze-thaw cycles were performed.
Protocol
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