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Détails du produit
- ProductSizeAmountActiveMax® Human CEACAM-5 / CD66E μBeads, premium grade (for cells)2.5 mg2.5×10⁷ beadsActiveMax® Human CEACAM-5 / CD66E μBeads, premium grade (for cells)10 mg (2.5 mg×4)1.0×10⁸ beads
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This product can be directly isolated using conventional column-free magnetic stand (eg. Cat. MB-01/ MB-02/ MB-03/ MB-04). Please note that due to its micron-scale size, it is not compatible with commercially available column-based (M/D/L) magnetic separation systems.
Product Description
ActiveMax® Human CEACAM-5 / CD66e μBeads, premium grade (for cells) are uniform, superparamagnetic beads of 5.5 µm in diameter immobilized with Human CEACAM-5 / CD66e protein expressed from human 293 cells (HEK293) and contains AA Lys 35 - Ala 685 (Accession # P06731-1).
ActiveMax® Human CEACAM-5 / CD66e μBeads, premium grade (for cells) are produced under sterile manufacturing conditions (ISO 5) , and no animal- or human-derived components are used throughout the production process. It is produced under our rigorous quality control system that includes a comprehensive set of tests including sterility and endotoxin tests.Application
ActiveMax® Human CEACAM-5 / CD66e μBeads, premium grade (for cells) are designed to stimulate in vitro CEACAM-5 / CD66e-specific CAR-T cells or UCAR-T cells, similar to the tumor cell lines that express human CEACAM-5 / CD66e antigen. It can be used as follows:
Evaluating the characteristics of CAR-T cells or UCAR-T cells.
In vitro expansion of CEACAM-5 / CD66e-specific CAR-T cells or UCAR-T cells.
In vitro enrichment of CEACAM-5 / CD66e-specific CAR-T cells or UCAR-T cells.Reconstitution
See Certificate of Analysis (CoA) for detailed instruction.
Storage
This product is stable in storage under the following conditions: -20˚C for 12 months in lyophilized state. -70°C for 3 months under sterile conditions after reconstitution.
Please protect from light and avoid repeated freeze-thaw cycles. Immediate use after reconstitution is highly recommended.
Sterility
Negative
Mycoplasma
Negative
Important Note
This product is for research use only and not intended for therapeutic or in vivo diagnostic use.
Formulation
Lyophilized in PBS with 0.1% HSA，pH 7.4. Trehalose is added as protectant before lyophilization.
ACRO Quality Management System
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Affichage des données
FACS Data
Assay of Human CEACAM-5 / CD66e Antibody on the μBeads surface by Flow cytometry. The Human CEACAM-5 / CD66e Antibody conjugated on the μBeads (Cat. No. MBS-C014) surface were fluorescently stained using PE anti-human CEACAM-5, Mouse Mab IgG2a Antibody, analyzed by flow cytometry (QC tested).
Protocol
Stimulate CEACAM-5 CAR-T Cells -Cytokines
CEACAM-5 CAR-T cells were stimulated with ActiveMax® Human CEACAM-5 μBeads (Cat. No. MBS-C014) for 24 h , and cell-free supernatants were harvested for evaluating IFN-γ、TNF-α、IL-4、IL-6 secretion by flow cytomtry. The results showed that CEACAM-5 CAR-T cells released significantly larger amounts of IFN-γ、TNF-α、IL-4、IL-6 into the supernatants in response to CEACAM-5 μBeads (Data is the average of 3 independent experiments, error bars ± SD).
Stimulate CEACAM-5 CAR-T Cells -Cytotoxic Molecules
CEACAM-5 CAR-T cells were stimulated with ActiveMax® Human CEACAM-5 μBeads (Cat. No. MBS-C014) for 24h, and cell-free supernatants were harvested for evaluating Granzyme B secretion by ELISA. The results showed that CEACAM-5 CAR-T cells released significantly larger amounts of Granzyme B into the supernatants in response to CEACAM-5 μBeads (Data is the average of 3 independent experiments, error bars ± SD).
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Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Carcinoembryonic antigen related cell adhesion molecule 5
- Catégorie：
- Nombre de médicaments répertoriés：
0 Détails
- Nombre de médicaments cliniques：
18 Détails
- Étape de développement la plus élevée：
Phase 3 Clinical
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