RPMI 8226/Luc Stable Cell Line Development Service

• Genetically modified cell lines best reflect MOA (Mechanism of Action)
• Higher activity and larger assay window for robust and reproducible cell-based bioassay
• Comprehensive application data to support assay development and validation
• Full tracible record, stringent quality control and validated cell passage stability
• Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
• Global commercial license assistance whenever regulatory filing is required

The RPMI 8226/Luc Stable Cell Line was engineered to express the firefly luciferase reporter. The luciferase activity was confirmed by the detection of luminescence signal.

• Used as target cells for luminescence-based cytotoxicity evaluation

Suspension

Puromycin (0.5 μg/mL)

RPMI-1640 + 10% FBS + L-Glutamine (2 mM)

Serum-free cell cryopreservation medium

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Frozen in liquid nitrogen.

Negative

Negative

See data sheet for detailed culturing and assay protocol.