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Product Specifications
Assay TypeSandwich-ELISAAnalyte4-1BB LigandFormat96T (8×12 strips)ReactivityHumanRegulatory StatusRUOSensitivity＜15.625 pg/mLStandard Curve Range15.625 pg/mL-500 pg/mLAssay Time2 hr 50 minSuitable Sample TypeFor the detection and quantitative determination of 4-1BB Ligand in human serum and cell culture supernatant.Sample volume100 μLProduct Overview
Human 4-1BB Ligand ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is used to detect and quantitatively determine GMP human 4-1BB Ligand residues in cell culture supernatants, serum, and plasma. The kit contains GMP human 4-1BB Ligand (ACROBiosystems, cat# GMP-41LH26) to ensure accurate assay results and is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages. It can also be used as a universal detection tool for the quantitative determination of Human 4-1BB Ligand.
Reconstitution
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeRES036-C01Pre-coated Anti-4-1BB Ligand Antibody Microplate1 plate (8×12 strips)RES036-C02Human 4-1BB Ligand Standard20 μgRES036-C03Biotin-Anti-4-1BB Ligand Antibody20 μgRES036-C04Streptavidin-HRP50 μLRES036-C0510×Washing Buffer50 mLRES036-C062×Dilution Buffer50 mLRES036-C07Substrate Solution12 mLRES036-C08Stop Solution7 mLACRO Quality Management System
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Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
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Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Tumor necrosis factor ligand superfamily member 9
- Catégorie：
- Nombre de médicaments répertoriés：
0 Détails
- Nombre de médicaments cliniques：
2 Détails
- Étape de développement la plus élevée：
Phase 2 Clinical
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