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Materials Provided
IDComponentsSizeBIS001-C01Pre-coated Human BCMA Protein Microplate1 plate (8×12 strips)BIS001-C02Anti-BCMA & CD3 Antibody Standard20 μgBIS001-C03Biotin-Human CD3E Protein20 μgBIS001-C04Streptavidin-HRP20 μgBIS001-C0510×Washing Buffer50 mLBIS001-C062×Dilution Buffer50 mLBIS001-C07Substrate Solution12 mLBIS001-C08Stop Solution7 mLProduct Overview
The kit is designed to analyze the ability of Bispecific Antibodies (BsAbs) to bridge BCMA (B-Cell Maturation Antigen, also known as Tumor necrosis factor receptor superfamily member 17 (TNFRSF17)) and CD3 (cluster of differentiation 3) for screening and profiling applications. This assay kit can determine if an anti-BCMA-anti-CD3 bispecific antibody binds to both targets simultaneously and bridges BCMA to CD3.
Storage
1. Store the unopened kit at 2-8°C upon receipt.
2. Locate the expiration date on the outer packaging and do not use reagents beyond their expiration date.
3. The opened kit should be stored according to the storage conditions listed in the Components Table. The shelf life of the opened kit is 30 days from the date of opening.
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Affichage des données
Typical Data
Please refer to DS document for the assay protocol.
Detection of anti-BCMA & CD3 antibodies by Bridging ELISA Assay.
Bispecific Human BCMA & CD3E Bridging ELISA Kit can specifically identify Anti-BCMA & CD3 Antibodies, with linear range of 0.010-0.317 nM (QC tested).
The kit shows favorable lot-to-lot consistency, with the RSD of EC50 less than 25%.
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