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Product Overview
Human HGF ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human HGF that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteHGFFormat96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity40 pg/mLAssay Time1 hr 45 minSample volume50 μLRange62.5 pg/mL-8000 pg/mLSample TypeCell Culture Supernatants, Plasma, Serum.NIBSC Code96/564Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human HGF in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow
Storage
Unopened kit should be stored at 2℃-8℃ upon receiving. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCRB012-C01Pre-coated Anti-HGF Antibody Microplate1 plateCRB012-C02Human HGF Standard20 μg×2CRB012-C03Biotin-Anti-HGF Antibody Con. Solution100 μLCRB012-C04Biotin-Antibody Dilution Buffer8 mLCRB012-C05Streptavidin-HRP Con. Solution500 μLCRB012-C06Streptavidin-HRP Dilution Buffer15 mLCRB012-C0720×Washing Buffer50 mLCRB012-C081×Dilution Buffer15 mL×2CRB012-C09Substrate Solution12 mLCRB012-C10Stop Solution6 mLACRO Quality Management System
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Affichage des données
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of HGF is less than 40.0 pg/mL.
Sample Values
Serum Sample: Twenty healthy serum samples were evaluated for the concentrations of human HGF in assay.
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Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Hepatocyte growth factor
- Catégorie：
- Nombre de médicaments répertoriés：
1 Détails
- Nombre de médicaments cliniques：
7 Détails
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