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Product Details
Product Overview
The Human IgE ELISA Kit is a ready‑to‑use immunoassay kit specifically designed for the quantification of native and human IgE in biological samples, including serum and plasma. Our ELISA kit offers multiple advantages:
Product Specifications
Assay TypeSandwich-ELISAAnalyteIgEFormat96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity50 pg/mLRange78 pg/mL-5000 pg/mLAssay Time2.75 hrSample volume50 μLSample TypeFor the quantitative determination of human lgE in serum, plasma.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for the quantification of native human IgE in serum, plasma.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA294-C01Pre-coated Anti-IgE Antibody Microplate1 plateCEA294-C02Human IgE Standard5 μg/mL×4CEA294-C03Biotin-Anti-IgE Antibody Con. Solution100 μLCEA294-C04Biotin-Antibody Dilution Buffer8 mLCEA294-C05Streptavidin-HRP Con. Solution500 μLCEA294-C06Streptavidin-HRP Dilution Buffer15 mLCEA294-C0720× Washing Buffer50 mLCEA294-C08Sample Dilution Buffer15 mL×2CEA294-C09Substrate Solution12 mLCEA294-C10Stop Solution6 mLACRO Quality Management System
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of IgE is less than 50 pg/mL.
Sample Values
Healthy donor serum samples (n=24) were evaluated for the concentrations of human IgE in the assay. The detectability rate of the samples was 100%, with a mean detectable concentration of 38.5 ng/mL and a concentration range of 157pg/mL~412 ng/mL.
Intra-Assay Statistics
Ten replicates of each of 3 samples containing different IgE concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three samples containing different concentrations of IgE were tested in independent assays. Acceptable criteria: CV<15%.
Recovery
Recombinant IgE was spiked into 3 human serum samples, and then analyzed. The average recovery of IgE for serum samples is 101.2%.
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