ELISA Assay Kit for Anti-PD-1 h-mAb in Mouse Serum

• Wide Coverage - This kit has been tested for multiple investigational PD-1 antibodies, as well as the commercially available Keytruda® and Opdivo®. There is no requirement for the species and subtype of the antibody.
• Easy Process - There is no longer a need to generate anti-drug antibodies for PK studies.
• Clean Result - The use of the biotinylated antibody in the competitive ELISA significantly alleviates the issue of background noise issue that often occurs in traditional ELISA method.

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

1. Unopened kit should be stored at -25~-15℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

The enclosed ELISA assay kit for anti-PD-1 h-mAb in mouse serum is based on an enzyme immunoassay (ELISA) between recombinant PD-1 protein (ECD) and a biotinylated anti-PD-1 monoclonal antibody. The method employs the principle of competitive ELISA, allowing quantification of monoclonal anti-PD-1 antibodies in mouse serum.

The assay only involves the following steps:

1. Coat the plate with human PD-1;

2. Add the mixture of your sample and biotinylated anti-PD-1 antibody;

3. Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate;

4. Record the OD readings and analyze the serum concentration.