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Product Details
Product Specifications
Assay TypeSandwich-ELISAAnalyteSCFFormat96T (8×12 strips)ReactivityHumanRegulatory StatusRUOSensitivity＜15.625 pg/mLStandard Curve Range15.625 pg/mL-1000 pg/mLAssay Time2 hr 50 minSuitable Sample TypeFor the detection and quantitative determination of GMP human SCF in human serum and cell culture supernates.Sample volume100 μLProduct Overview
Human SCF ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is used to detect and quantitatively determine GMP human SCF residues in cell culture supernatants, serum, and plasma. It contains GMP human SCF (ACROBiosystems, cat# GMP-SCFH25) to ensure accurate assay results and is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages. It can also be used as a universal detection tool for the quantitative determination of human SCF.
Reconstitution
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeRES006-C01Pre-coated Anti-SCF Antibody Microplate1 plate (8×12 strips)RES006-C02Human SCF Standard20 μgRES006-C03Biotin-Anti-SCF Antibody20 μgRES006-C04Streptavidin-HRP50 μLRES006-C0510×Washing Buffer50 mLRES006-C062×Dilution Buffer50 mLRES006-C07Substrate Solution12 mLRES006-C08Stop Solution7 mLACRO Quality Management System
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
Dilution Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human SCF were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Intra-Assay Statistics
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.
Inter-Assay Statistics
Three samples of known concentration were tested in three separate assays to assess inter-assay precision.
Recovery
Five parts of blank T cell culture supernatant were added with different concentrations of human SCF, and the T cell culture supernatant without human SCF was used as background to calculate the recovery rate. The range of the recovery rate is 90.9-101.3%, and the average recovery is 95.3%.
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