Détails du produit
Product Specifications
Assay TypeSandwich-ELISAAnalyteTrypLEFormat96T (8×12 strips)Regulatory StatusRUOSensitivity<12.5 pg/mLStandard Curve Range12.5 pg/mL-400 pg/mLAssay Time2 hrSuitable Sample TypeFor the detection and quantitative determination of TrypLE in cell culture sample.Sample volume100 μLProduct Overview
TrypLE (Trypsin Like Enzyme) is an animal origin-free, recombinant enzyme. It's an important raw material from the acquisition of early primary cells to the subsequent passage and digestion of adherent cells, and then to large-scale cell factory or microcarrier reactor culture.The amount of trypLE should be strictly controlled when digesting cells, and the detection of trypLE residues is also an important link to ensure the normal growth of cells.Because a large amount of trypLE was used for digestion in the culture stage, it is necessary to test for trypLE of the downstream part of the production.
Application
The kit is developed for the detection and quantitative determination of residual trypLE in bio-manufacturing down-stream processing fast and reliable. The assay is perfectly suited for process development, in-process control and final quality control.
It is for research use only.
Storage
1. Store the unopened kit at 2-8°C upon receipt.
2. Locate the expiration date on the outer packaging and do not use reagents beyond their expiration date.
Materials Provided
IDComponentsSizeRES057-C01Pre-coated Anti-TrypLE Antibody Microplate1 plate (8×12 strips)RES057-C02TrypLE Standard15 μgRES057-C03HRP-Anti-TrypLE Antibody15 μgRES057-C0410×Washing Buffer50 mLRES057-C052×Dilution Buffer50 mLRES057-C06Substrate Solution12 mLRES057-C07Stop Solution7 mLACRO Quality Management System
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Affichage des données
Typical Data
Please refer to DS document for the assay protocol.

For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
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