SARS-CoV-2 Spike protein RBD-Coated Plates, Clear, 96-Well

Product Overview

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Application

This SARS-CoV2 Spike protein RBD- Coated Plate is intended for ELISA Assay and Biopannning.

Features

1. Flexible experimental design because of the plate consist with detachable 8-well strips.
2. Save your experiment time by pre-coated and blocked.
3. No denaturing of the protein component of a conjugate upon binding.
4. Ideal for binding anti-SARS2-CoV-2 S1 antibodies or ACE2 protein that typically exhibit poor binding to polystyrene.

Storage

The unopened plate should be stored at 2°C to 8°C,The expiry date of the plate is 12 months. Once opened, place unused plates in a resealable bag with desiccant and store at 2°C to 8°C, The shelf life is 1 month from the date of opening.

Assay Principles

Example ELISA Procedure.

Your experiment will include 4 simple steps:

a) Add your antibody samples or ACE2 protein to the plate, incubation for 1 hour and wash the plate.

b) Add a diluted Detection antibody to the plate and incubation.

c) Wash the plate and next add TMB or other colorimetric HRP substrate.

d) Stop the substrate reaction by add diluted acid, and Read OD at 450 nm, the OD Value reflects the amount of antibody bound.

Example Biopanning Procedure

Biopanning is an affinity selection technique which selects for peptides that binds to a given target. In the capturing step, just add the antigen couple beads to your phage library. Simply use a magnet to separate the bound phages from the unbound ones. More efficient and time saving compared to the plate based capture.

Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10−14 mol/L, the Biotinylated molecules can bind to the SA irreversibly. The SARS-CoV2 Spike protein RBD-Coated Plates are immobilized with biotinylated SARS-CoV2 Spike protein RBD to a Streptavidin tetramer protein coated plate, which is easy to use and widely available for applications.