Product Details
Synonyms
Activin E
Source
Human Activin E Protein, His Tag (INE-H5143) is expressed from E. coli cells. It contains AA Thr 237 - Ser 350 (Accession # P58166).
Predicted N-terminus: Met
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 14.5 kDa. The protein migrates as 15-16 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM Citric acid, pH2.2 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE

Human Activin E Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA

Immobilized Human Activin E Protein, His Tag (Cat. No. INE-H5143) at 5 μg/mL (100 μL/well) can bind Human / Cynomolgus Activin RIIA / ACVR2A Protein, Fc Tag(Cat. No. ACA-H5269) with a linear range of 0.004-0.06 μg/mL (QC tested).
Protocol
Bioactivity-CELL BASE

Human Activin E Protein, His Tag (Cat. No. INE-H5143) induced ALK-7 reporter activity in HEK293 cells. The EC50 for this effect is 0.9882 ng/mL (Routinely tested).
Protocol
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