Human Activin E Protein, His Tag

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Cat. No. / Size
Price
Qty
INE-H5143-100ug
$420.00
INE-H5143-1mg
$3360.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Activin E

  • Source

    Human Activin E Protein, His Tag (INE-H5143) is expressed from E. coli cells. It contains AA Thr 237 - Ser 350 (Accession # P58166).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    Activin E Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 14.5 kDa. The protein migrates as 15-16 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM Citric acid, pH2.2 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Activin E SDS-PAGE

    Human Activin E Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     Activin E ELISA

    Immobilized Human Activin E Protein, His Tag (Cat. No. INE-H5143) at 5 μg/mL (100 μL/well) can bind Human / Cynomolgus Activin RIIA / ACVR2A Protein, Fc Tag(Cat. No. ACA-H5269) with a linear range of 0.004-0.06 μg/mL (QC tested).

    Protocol
  • Bioactivity-CELL BASE

     Activin E CELL

    Human Activin E Protein, His Tag (Cat. No. INE-H5143) induced ALK-7 reporter activity in HEK293 cells. The EC50 for this effect is 0.9882 ng/mL (Routinely tested).

    Protocol

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Background

Activins are one of the three distinct subclasses within the greater Transforming growth factor β superfamily. The members of the activin subclass include activin A, activin B, activin C, activin E, and the more distant members myostatin and GDF-11. Activin E is a homodimer of INHBE secreted by the liver that plays a crucial role in regulating metabolic homeostasis particularly in lipid metabolism and energy homeostasis.

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