Biotinylated Human ALK-7 Protein, Fc,Avitag™ (MALS verified)

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Cat. No. / Size
Price
Qty
AL7-H82F3-25ug
$360.00
AL7-H82F3-200ug
$1420.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    ALK-7, ACTR-IC, ACVR1C

  • Source

    Biotinylated Human ALK-7 Protein, Fc,Avitag (AL7-H82F3) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Glu 113 (Accession # Q8NER5-1).

    Predicted N-terminus: Glu 21

    Request for sequence
  • Molecular Characterization

    ALK-7 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 38.2 kDa. The protein migrates as 45-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    ALK-7 SDS-PAGE

    Biotinylated Human ALK-7 Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    ALK-7 SEC-MALS

    The purity of Biotinylated Human ALK-7 Protein, Fc,Avitag (Cat. No. AL7-H82F3) is more than 90% and the molecular weight of this protein is around 85-110 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     ALK-7 ELISA

    Immobilized Biotinylated Human ALK-7 Protein, Fc,Avitag (Cat. No. AL7-H82F3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Rabbit anti-Homo sapiens (Human) ACVR1C Polyclonal antibody, Rabbit IgG) with a linear range of 0.0005-0.125 μg/mL (QC tested).

    Protocol

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Background

ALK-7, also known as ACVR1C , is a Type I receptor for the TGF-beta family of signaling molecules. Upon ligand binding, it initiates intracellular signaling by phosphorylating cytoplasmic SMAD transcription factors. These activated SMADs then translocate to the nucleus to regulate gene expression, either directly or in complex with other transcription factors.

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