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Cynomolgus Alpha-Synuclein Protein, His Tag (MALS verified)

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Numéro de produit/spécification
Prix
Qté
ALN-C52H5-200ug
$185.00
ALN-C52H5-1mg
$695.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    SNCA, NACP, PARK1, alpha-Synuclein

  • Source

    Cynomolgus Alpha-Synuclein, His Tag (ALN-C52H5) is expressed from E. coli cells. It contains AA Met 1 - Ala 140 (Accession # P61142-1).

    Predicted N-terminus: Met 1

    Request for sequence
  • Molecular Characterization

    Alpha-Synuclein Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 16.4 kDa. The protein migrates as 18-20 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 100 mM NaAC, pH7.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    Alpha-Synuclein SDS-PAGE

    Cynomolgus Alpha-Synuclein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    Alpha-Synuclein SEC-MALS

    The purity of Cynomolgus Alpha-Synuclein, His Tag (Cat. No. ALN-C52H5) is more than 90% and the molecular weight of this protein is around 14-20 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     Alpha-Synuclein ELISA

    Immobilized Cynomolgus Alpha-Synuclein, His Tag (Cat. No. ALN-C52H5) at 1 μg/mL (100 μL/well) can bind Anti-Synucelin alpha/beta Monoclonal Antibody with a linear range of 0.004-0.25 μg/mL (QC tested).

    Protocol

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Introduction de fond

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca2+ release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity.

Progrès de frontière

 
Progrès du développement médicamenteux
  • Nom anglais:

    Synuclein alpha

  • Catégorie:

  • Nombre de médicaments répertoriés:

    0 Détails

  • Nombre de médicaments cliniques:

    25 Détails

  • Étape de développement la plus élevée:

    Phase 3 Clinical

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