Human Angiopoietin-like 3 / ANGPTL3 Protein, His Tag

Synonyms

ANGPTL3, ANGPT5, ANG-5, Angiopoietin-5, FHBL2

Source

Human Angiopoietin-like 3, His Tag (AN3-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ser 17 - Glu 460 (Accession # Q9Y5C1-1).

Predicted N-terminus: Ser 17

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin-like convertase cleavage site, 221-RAPR-224, and will be partially processed into N and C-terminal fragment with calculated MW of 24.3 kDa and 29.4 kDa respectively. The protein migrates as 55-70 kDa (full lengh), 31-33 kDa (C-terminal fragment) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, 0.05% CHAPS, pH7.4 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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