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Product Details
Synonyms
Apolipoprotein E, APOE, Apo-E
Source
Mouse Apolipoprotein E Protein, His Tag (APE-M52H5) is expressed from human 293 cells (HEK293). It contains AA Glu 19 - Gln 311 (Accession # P08226).
Predicted N-terminus: Glu 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 35.9 kDa. The protein migrates as 14 kDa and 35 kDa under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Mouse Apolipoprotein E Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Mouse Apolipoprotein E Protein, His Tag (Cat. No. APE-M52H5) is more than 90% and the molecular weight of this protein is around 135-155 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Human LDL R, Fc Tag at 5 μg/mL (100 μL/well) can bind Mouse Apolipoprotein E Protein, His Tag (Cat. No. APE-M52H5) with a linear range of 0.02-2.5 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Mouse Apolipoprotein E Protein, His Tag (Cat. No. APE-M52H5) immobilized on CM5 Chip can bind Mouse LDL R, His Tag (Cat. No. LDR-M52H8) with an affinity constant of 73.4 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Apolipoprotein E
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
3 Details
- Highest Development Stage：
Phase 2 Clinical
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