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Human B7-H3 (2Ig) Protein, His Tag AIDD-Ready protein is designed for direct use in SPR assays. It is supplied in a sugar-free, glycerol-free formulation to eliminate refractive index interference in SPR. No desalting or buffer exchange is required before use, simplifying the SPR workflow and supporting high-throughput experimental validation to accelerate the transition from AI prediction to wet-lab validation.
B7-H3, CD276, B7 homolog 3
Human B7-H3 (2Ig) Protein, His Tag (B73-H52E2AI) is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Pro 245 (Accession # Q5ZPR3-2).
Predicted N-terminus: Leu 29

This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 25.2 kDa. The protein migrates as 30-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Formulated in a proprietary trehalose-free buffer, supplied ready-to-use for SPR/BLI analysis - no buffer exchange required.
For detailed formulation, please refer to the batch-specific COA.
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.