Product Details
Synonyms
4Ig-B7-H3, B7-H3, CD276, PSEC0249, UNQ309, PRO352, B7 homolog 3
Source
Human B7-H3 (4Ig), Fc Tag (B7B-H5258) is expressed from human 293 cells (HEK293). It contains AA Gly 27 - Thr 461 (Accession # Q5ZPR3-1).
Predicted N-terminus: Gly 27
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 73.1 kDa. The protein migrates as 100-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Human B7-H3 (4Ig), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS

The purity of Human B7-H3 (4Ig), Fc Tag (Cat. No. B7B-H5258) is more than 85% and the molecular weight of this protein is around 155-190 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Human B7-H3 (4Ig), Fc Tag (Cat. No. B7B-H5258) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human B7-H3 / B7-H3 (4Ig) Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (QC tested).
Protocol
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FAQ
- Product
What is the difference between Human B7-H3 and Human B7-H3 (4Ig)?
The Human B7-H3 protein with an expression range of Leu29–Pro245 contains a single pair of immunoglobulin-like V and C domains. The product with an expression range of Gly27–Thr461, named B7-H3 (4Ig), includes the entire extracellular region.
Background
Recent Advances
- English Name:
CD276 antigen
- Category:
- Approved Drugs:
0 Details
- Drugs in Clinical Trials:
72 Details
- Highest Development Stage:
NDA/BLA



















