Human B7-H3 (4Ig) / B7-H3b Protein, Fc Tag (MALS verified)

Customer Reviews
Cat. No. / Size
Price
Qty
B7B-H5258-100ug
$355.00
B7B-H5258-1mg (500ug X 2)
$2300.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    4Ig-B7-H3, B7-H3, CD276, PSEC0249, UNQ309, PRO352, B7 homolog 3

  • Source

    Human B7-H3 (4Ig), Fc Tag (B7B-H5258) is expressed from human 293 cells (HEK293). It contains AA Gly 27 - Thr 461 (Accession # Q5ZPR3-1).

    Predicted N-terminus: Gly 27

    Request for sequence
  • Molecular Characterization

    B7-H3 (4Ig) Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 73.1 kDa. The protein migrates as 100-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

Customers Also Viewed

Performance Data

  • SDS-PAGE

    B7-H3 (4Ig) SDS-PAGE

    Human B7-H3 (4Ig), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    B7-H3 (4Ig) SEC-MALS

    The purity of Human B7-H3 (4Ig), Fc Tag (Cat. No. B7B-H5258) is more than 85% and the molecular weight of this protein is around 155-190 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     B7-H3 (4Ig) ELISA

    Immobilized Human B7-H3 (4Ig), Fc Tag (Cat. No. B7B-H5258) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human B7-H3 / B7-H3 (4Ig) Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (QC tested).

    Protocol

Customer Reviews
Writing Reviews

FAQ

  • Product

    What is the difference between Human B7-H3 and Human B7-H3 (4Ig)?

    The Human B7-H3 protein with an expression range of Leu29–Pro245 contains a single pair of immunoglobulin-like V and C domains. The product with an expression range of Gly27–Thr461, named B7-H3 (4Ig), includes the entire extracellular region.

Background

Human B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for the regulation of immune responses. Other family members include B7-1, B7-2, B7-H1/PD-L1, B7-H2, and PD-L2. B7 family proteins are type I transmembrane immunoglobulin (Ig) superfamily members that contain extracellular Ig V‑like and Ig C‑like domains with a short cytoplasmic tail. Termed 4IgB7-H3 or B7-H3b, this molecule has two additional Ig-like domains (one V‑type and one C‑type) and shows a ubiquituous expression pattern.

Recent Advances

 
Drug Development Progress
  • English Name:

    CD276 antigen

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    72 Details

  • Highest Development Stage:

    NDA/BLA

Drug Candidate Licensing
More
  • Basic Product Information
  • Product Details
  • Customers Also Viewed
  • Performance Data
  • Customer Reviews
  • FAQ
  • Background