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PE-Labeled Human B7-H3 (4Ig) / B7-H3b Protein, His Tag (Site-specific conjugation)

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Numéro de produit/spécification
Prix
Qté
B7B-HP2H9-25tests
$345.00
B7B-HP2H9-200tests
$2140.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    4Ig-B7-H3, B7-H3, CD276, PSEC0249, UNQ309, PRO352, B7 homolog 3

  • Source

    PE-Labeled Human B7-H3 (4Ig), His Tag (B7B-HP2H9) is produced via site-specific conjugation of PE to Human B7-H3 (4Ig), His Tag under optimal conditions with a proprietary technology. Human B7-H3 (4Ig), His Tag is expressed from human 293 cells (HEK293). It contains AA Gly 27 - Thr 461 (Accession # Q5ZPR3-1).

    Predicted N-terminus: Gly 27

    Request for sequence

  • Molecular Characterization

    B7-H3 (4Ig) Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 50.3 kDa.

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Application

    Please note that this product is NOT compatible to streptavidin detection system.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Affichage des données

  • Bioactivity-FACS

     B7-H3 (4Ig) FACS

    5e5 of anti-B7-H3 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human B7-H3 (4Ig), His Tag (Cat. No. B7B-HP2H9) and negative control protein respectively. PE signal was used to evaluate the binding activity (QC tested).

    Protocol

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FAQ

  • Product

    What is the difference between Human B7-H3 and Human B7-H3 (4Ig)?

    The Human B7-H3 protein with an expression range of Leu29–Pro245 contains a single pair of immunoglobulin-like V and C domains. The product with an expression range of Gly27–Thr461, named B7-H3 (4Ig), includes the entire extracellular region.

Introduction de fond

Human B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for the regulation of immune responses. Other family members include B7-1, B7-2, B7-H1/PD-L1, B7-H2, and PD-L2. B7 family proteins are type I transmembrane immunoglobulin (Ig) superfamily members that contain extracellular Ig V‑like and Ig C‑like domains with a short cytoplasmic tail. Termed 4IgB7-H3 or B7-H3b, this molecule has two additional Ig-like domains (one V‑type and one C‑type) and shows a ubiquituous expression pattern.

Progrès de frontière

 
Progrès du développement médicamenteux
  • Nom anglais:

    CD276 antigen

  • Catégorie:

  • Nombre de médicaments répertoriés:

    0 Détails

  • Nombre de médicaments cliniques:

    72 Détails

  • Étape de développement la plus élevée:

    NDA/BLA

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