Save Product
Product Details
Synonym
TNFRSF17, CD269, BCM, BCMA
Source
Biotinylated Human BCMA, Fc,Avitag, premium grade (BC7-H82F0) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 54 (Accession # NP_001183.2).
Predicted N-terminus: Met 1
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage.
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 33.8 kDa. The protein migrates as 40-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Sterility
Negative
Mycoplasma
Negative
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Customers Also Viewed
Performance Data
SDS-PAGE
Biotinylated Human BCMA, Fc,Avitag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human BCMA, Fc,Avitag, premium grade (Cat. No. BC7-H82F0) is more than 95% and the molecular weight of this protein is around 65-85 kDa verified by SEC-MALS.
Report
Bioactivity-FACS
2e5 of anti-BCMA CAR-293 cells were stained with 100 μL of 3 μg/mL of Biotinylated Human BCMA, Fc,Avitag, premium grade (Cat. No. BC7-H82F0) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).
Protocol
Bioactivity-ELISA
Immobilized Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) at 2 μg/mL (100 μL/well) can bind Biotinylated Human BCMA, Fc,Avitag, premium grade (Cat. No. BC7-H82F0) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized Anti-Human BCMA Mab at 2 μg/mL (100 μL/well) can bind Biotinylated Human BCMA, Fc,Avitag, premium grade (Cat. No. BC7-H82F0) with a linear range of 0.1-3 ng/mL (Routinely tested).
Protocol
Quantitative Analysis of Bispecific T-cell Engager (CD3 X BCMA) in human serum.
Immobilized Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-H52W1) at 2 μg/mL, add increasing concentrations of Bispecific T cell Engager (CD3 X BCMA) in 10% human serum and then add Biotinylated Human BCMA, Fc,Avitag, premium grade (Cat. No. BC7-H82F0) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL (Routinely tested).
Immobilized Human APRIL, His,Flag Tag, active trimer (Cat. No. APL-H52D1) at 1 μg/mL (100 μL/well) can bind Biotinylated Human BCMA, Fc,Avitag, premium grade (Cat. No. BC7-H82F0) with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
Evaluation of CAR expression
FACS analysis of anti-BCMA CAR Expression
Human T cells were transfected with anti-BCMA CAR and cultured for 3 days. Three days post-transfection, 1e6 cells were first incubated with 50 μL Biotinylated Human BCMA, Fc,Avitag, premium grade (Cat. No. BC7-H82F0, 8 μg/mL), washed and then stained with PE Streptavidin and analyzed by flow cytometry. (Data are kindly provided by PREGENE Biopharma)
Customer Reviews Writing Reviews
Background
Recent Advances
Drug Development Progress- English Name：
B-cell maturation protein
- Category：
- Approved Drugs：
7 Details
- Drugs in Clinical Trials：
117 Details
- Highest Development Stage：
Phase 3 Clinical
Contact Us
Drug Candidate Licensing