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Product Details
Synonym
TNFRSF17, CD269, BCM, BCMA
Source
FITC-Labeled Human BCMA, Fc Tag (Cat. No. BCA-HF254) is expressed from human HEK293 cells. It contains AA Met 1 - Ala 54 (Accession # Q02223-1). It is the FITC labeled form of Human BCMA, Fc Tag (Cat. No. BC7-H5254).
Predicted N-terminus: Met 1
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 31.97 kDa. The protein migrates as 35-44 kDa under reducing (R) condition, and 60-75 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Conjugate
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
Protein Ratio
The FITC to protein molar ratio is 1.5-3.
Purity
>92% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
FITC-Labeled Human BCMA Protein, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 92% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human BAFF, Fc Tag at 10 μg/mL (100 μL/well) can bind FITC-Labeled Human BCMA Protein, Fc Tag (Cat. No. BCA-HF254) with a linear range of 0.02-1.25 μg/mL (QC tested).
Protocol
Bioactivity-FACS
2e5 of anti-BCMA CAR-293 cells were stained with 100 μL of 3 μg/mL of FITC-Labeled Human BCMA Protein, Fc Tag (Cat. No. BCA-HF254) and negative control protein respectively. FITC signal was used to evaluate the binding activity (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
B-cell maturation protein
- Category：
- Approved Drugs：
7 Details
- Drugs in Clinical Trials：
117 Details
- Highest Development Stage：
Phase 3 Clinical
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