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Human Betacellulin Protein, Research GradeResearch use only

  • This product is designed for academic and scientific research institutions.
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Numéro de produit/spécification
Prix
Qté
BEN-H5117-20ug
$200.00
BEN-H5117-50ug
$345.00
BEN-H5117-1mg (200ug X 5)
$2240.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Features and Advantages

    1. Native Conformation: Native sequences, tag-free and natural function.
    2. Reliable Activity: Biological activity calibrated against WHO/NIBSC standards.
    3. Stringent Quality Control: Protein content, purity, and cell-based bioactivity testing for each batch.
    4. Lowest Endotoxin level (<0.01 EU/ug).
    5. Safety Assurance: Sterile filtration through 0.2 μm membrane.
    6. AOF: Animal origin-free raw materials throughout the production process.
  • Synonyms

    betacellulin, BTC

  • Source

    Human Betacellulin Protein, Research Grade (BEN-H5117) is expressed from E. coli cells. It contains AA Asp 32 - Gln 118 (Accession # P35070-1).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    Betacellulin Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries no "tag".

    The protein has a calculated MW of 9.9 kDa. The protein migrates as 14 kDa±3 kDa under reducing (R) condition, and 14 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM Citric acid, pH2.2 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process
  • Quality Description

    ACRO's Research-grade products are suitable for a wide range of cell culture applications, particularly for research use in academic institutions. These products are sterilized by filtration, followed by lyophilization where applicable. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. Biological activity is calibrated against WHO/NIBSC standards when available.
    ACRO's Premium-grade (Pre-GMP) products are characterized by their high quality and enhanced safety profiles, making them ideal for early-stage discovery and manufacturing processes in cell therapy companies. A key advantage is their seamless transition to corresponding GMP-grade versions. Biological activity is calibrated against WHO/NIBSC standards when available. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. In addition, rigorous testing is conducted to ensure the absence of mycoplasma, HCD, and HCP, thereby guaranteeing product safety.

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  • SDS-PAGE

    Betacellulin SDS-PAGE

    Human Betacellulin Protein, Research Grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-CELL BASE

     Betacellulin CELL

    Human Betacellulin Protein, Research Grade (Cat. No. BEN-H5117) stimulates Human EGF R (Luc) HEK293 Reporter Cell. The specific activity of Human Betacellulin Protein, Research Grade is > 3.50 x 10^3 U/mg (QC tested).

    Protocol

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Introduction de fond

Betacellulin (BTC) is a member of the epidermal growth factor family that is comprised of at least ten proteins including EGF, TGF-alpha, amphiregulin, HB-EGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or more EGF structural units in their extracellular domain. Betacellulin is expressed predominantly in the human pancreas and induces the differentiation of a pancreatic acinar cell line (AR42J) into insulin-secreting cells, and signals through EGF receptor and ERBB4. It activates ERK and AKT pathways, which induces neural stem cell proliferation and prevents spontaneous differentiation in culture.

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