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PE-Labeled Human Carbonic Anhydrase IX / CA9 (38-414) Protein, His Tag (Site-specific conjugation)

Numéro de produit/spécification
Prix
Qté
CA9-HP2H6-25tests
$360.00
CA9-HP2H6-200tests
$2245.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    CAIX, CA9, CA-IX, G250, MN, P54, 58N, pMW1

  • Source

    PE-Labeled Human Carbonic Anhydrase IX (38-414), His Tag (CA9-HP2H6) is produced via site-specific conjugation of PE to Human Carbonic Anhydrase IX (38-414), His Tag under optimal conditions with a proprietary technology. Human Carbonic Anhydrase IX (38-414), His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 38 - Asp 414 (Accession # Q16790-1).

    Predicted N-terminus: Gln 38

    Request for sequence

  • Molecular Characterization

    Carbonic Anhydrase IX Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 44.5 kDa.

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Application

    Please note that this product is NOT compatible to streptavidin detection system.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Affichage des données

  • Bioactivity-FACS

     Carbonic Anhydrase IX FACS

    5e5 of anti-Carbonic Anhydrase IX CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human Carbonic Anhydrase IX (38-414), His Tag (Cat. No. CA9-HP2H6) and negative control protein respectively. PE signal was used to evaluate the binding activity (QC tested).

    Protocol

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Introduction de fond

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes. CAs form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. The active site of most carbonic anhydrases contains a zinc ion. There are at least five distinct CA families (α, β, γ, δ and ε).
Carbonic anhydrase 9 (CA9 / CAIX) is also known as Membrane antigen MN (MN), Renal cell carcinoma-associated antigen G250, which belongs to the alpha-carbonic anhydrase family. CA9 / CAIX with an optimal activity at pH 6.49. Reversible hydration of carbon dioxide. CA IX participates in pH regulation. CA9 may be involved in the control of cell proliferation and transformation. CA-IX appears to be a novel specific biomarker for a cervical neoplasia.

Progrès de frontière

 
Progrès du développement médicamenteux
  • Nom anglais:

    Carbonic anhydrase IX

  • Catégorie:

  • Nombre de médicaments répertoriés:

    0 Détails

  • Nombre de médicaments cliniques:

    16 Détails

  • Étape de développement la plus élevée:

    Phase 3 Clinical

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