Human CD209 / DC-SIGN Protein, His Tag

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Cat. No. / Size
Price
Qty
CD9-H5246-100ug
$395.00
CD9-H5246-1mg (500ug X 2)
$2615.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    DC-SIGN, CD209, DC-SIGN1, CLEC4L

  • Source

    Human CD209, His Tag (CD9-H5246) is expressed from human 293 cells (HEK293). It contains AA Gln 59 - Ala 404 (Accession # Q9NNX6-1).

    Predicted N-terminus: His

    Request for sequence
  • Molecular Characterization

    CD209 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 41.3 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    CD209 SDS-PAGE

    Human CD209, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Bioactivity-ELISA

     CD209 ELISA

    Immobilized SARS-CoV-2 S1 protein, Fc Tag (Cat. No. S1N-C5255) at 5 μg/mL (100 μL/well) can bind Human CD209, His Tag (Cat. No. CD9-H5246) with a linear range of 0.039-0.625 μg/mL (QC tested).

    Protocol

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Background

CD209 is also known as CLEC4L, DC-SIGN and CD209 antigen. Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells.

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