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Détails du produit
Synonyms
FLJ18683, T3E, TCRE, CD3E, CD3-epsilon
Source
Alexa Fluor 488-Labeled Mouse CD3 epsilon Protein, Fc Tag (CDE-MA256) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 108 (Accession # P22646). It is the Alexa Fluor™ 488 labeled form of Mouse CD3E, Fc Tag (Cat. No. CDE-M5256).
Predicted N-terminus: Asp 23
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 36 kDa. The protein migrates as 40-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Conjugate
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with AF488 using standard chemical labeling method. The residual AF488 is removed by molecular sieve treatment during purification process.
Protein Ratio
The AF488 to protein molar ratio is 1.5-2.5.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Alexa Fluor 488-Labeled Mouse CD3 epsilon Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Alexa Fluor 488-Labeled Mouse CD3 epsilon Protein, Fc Tag (Cat. No. CDE-MA256) at 5 μg/mL (100 μL/well) can bind CD3e Monoclonal Antibody with a linear range of 1-63 ng/mL (QC tested).
Protocol
Immobilized Alexa Fluor 488-Labeled Mouse CD3 epsilon Protein, Fc Tag (Cat. No. CDE-MA256) at 5 μg/mL (100 μL/well) can bind CD3e Monoclonal Antibody with a linear range of 1-63 ng/mL (Routinely tested). Labeling with fluorescent dyes did not affect their activity.
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
T-cell surface glycoprotein CD3 epsilon chain
- Catégorie：
- Nombre de médicaments répertoriés：
4 Détails
- Nombre de médicaments cliniques：
7 Détails
- Étape de développement la plus élevée：
Approved
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