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Human CD34 Protein, His Tag

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Numéro de produit/spécification
Prix
Qté
CD4-H52H9-100ug
$410.00
CD4-H52H9-1mg (500ug X 2)
$2945.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    CD34, RP11-328D5.2

  • Source

    Human CD34, His Tag (CD4-H52H9) is expressed from human 293 cells (HEK293). It contains AA Ser 32 - Thr 290 (Accession # P28906-1).

    Predicted N-terminus: Ser 32

    Request for sequence
  • Molecular Characterization

    CD34 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 29.4 kDa. The protein migrates as 67-96 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    CD34 SDS-PAGE

    Human CD34, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Bioactivity-ELISA

     CD34 ELISA

    Immobilized Human CD34, His Tag (Cat. No. CD4-H52H9) at 1 μg/mL (100 μL/well) can bind Mouse Anti-Human CD34 Antibody (3B10E5), Mouse IgG1 | Mouse Kappa with a linear range of 0.1-4 ng/mL (QC tested).

    Protocol

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Introduction de fond

CD34 molecule is a cluster of differentiation molecule present on certain cells within the human body. It is a cell surface glycoprotein and functions as a cell-cell adhesion factor. It may also mediate the attachment of stem cells to bone marrow extracellular matrix or directly to stromal cells. As a ~ 110 kDa monomeric cell surface antigen, CD34 is highly glycosylated with nine potential N-linked and numerous potential O-linked glycosylation sites in its extracellular domain. The CD34 protein is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early hematopoietic and vascular-associated tissue. CD34 is also an important adhesion molecule and is required for T cells to enter lymph nodes. It is expressed on lymph node endothelia whereas the L-selectin to which it binds is on the T cell. It was indicated that CD34 is a phosphorylation target for activated PKC, and couples to the hematopoietic adapter protein CrkL, which were involved in CD34 signaling pathways. CD34 is abberantly expressed in many kinds of tumors and is implicated in leukemogenesis.

Progrès de frontière

 
Progrès du développement médicamenteux
  • Nom anglais:

    Hematopoietic progenitor cell antigen CD34

  • Catégorie:

  • Nombre de médicaments répertoriés:

    1 Détails

  • Nombre de médicaments cliniques:

    12 Détails

  • Étape de développement la plus élevée:

    Approved

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