APC-Labeled Human CD3 epsilon&CD3 gamma Heterodimer Protein, Fc,His Tag&Fc,Flag Tag

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CDG-HA2W3-25ug
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CDG-HA2W3
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Synonyms

CD3 epsilon & CD3 gamma, CD3E & CD3G

Source

APC-Labeled Human CD3E & CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (CDG-HA2W3) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 & Gln 23 - Ser 116 (Accession # P07766-1 & P09693-1). It is the APC labeled form of Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-H52W9).

Predicted N-terminus: Asp 23 & Gln 23

Molecular Characterization

APC-Labeled Human CD3E & CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 39.2 kDa (CD3E) and 38.6 kDa (CD3G). The subunit CD3E carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The subunit CD3G carries a human IgG1 Fc fragment at the C-terminus, followed by a flag tag.

Conjugate

APC

Excitation Wavelength: 640 nm

Emission Wavelength: 661 nm

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background

T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.