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Détails du produit
Synonyms
CD40LG, CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39
Source
Mouse CD40 Ligand, His,Flag Tag (CDL-M52D5) is expressed from human 293 cells (HEK293). It contains AA Gly 115 - Leu 260 (Accession # P27548-1).
Predicted N-terminus: His
Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.
The protein has a calculated MW of 50.5 kDa. The protein migrates as 45-55 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Mouse CD40 Ligand, His,Flag Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Mouse CD40 Ligand, His,Flag Tag (Cat. No. CDL-M52D5) is more than 95% and the molecular weight of this protein is around 50-66 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Mouse CD40 Ligand, His,Flag Tag (Cat. No. CDL-M52D5) at 2 μg/mL (100 μL/well) can bind Mouse CD40, Fc Tag (Cat. No. TN5-M5259) with a linear range of 1-32 ng/mL (QC tested).
Protocol
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Introduction de fond
CD40 ligand binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In general, CD40 ligand plays the role of a costimulatory molecule and induces activation in APC in association with T cell receptor stimulation by MHC molecules on the APC. In total CD40 ligand has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD40 ligand regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper IgM syndrome.
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
CD40 ligand
- Catégorie：
- Nombre de médicaments répertoriés：
0 Détails
- Nombre de médicaments cliniques：
14 Détails
- Étape de développement la plus élevée：
Phase 3 Clinical
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