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Human CD43 / leukosialin Protein, His Tag (MALS & SPR verified)

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Numéro de produit/spécification
Prix
Qté
CD3-H52H9-100ug
$380.00
CD3-H52H9-1mg
$2380.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    leukosialin, sialophorin, GPL115, Galactoglycoprotein, GALGP, Leukocyte sialoglycoprotein

  • Source

    Human CD43, His Tag (CD3-H52H9) is expressed from human 293 cells (HEK293). It contains AA Ser 20 - Arg 253 (Accession # P16150-1 ).

    Predicted N-terminus: Ser 20

    Request for sequence
  • Molecular Characterization

    CD43 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 25.3 kDa. The protein migrates as 45-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    CD43 SDS-PAGE

    Human CD43, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    CD43 SEC-MALS

    The purity of Human CD43, His Tag (Cat. No. CD3-H52H9) is more than 90% and the molecular weight of this protein is around 38-48 kDa verified by SEC-MALS.

    Report
  • Bioactivity-SPR

     CD43 SPR

    Human Siglec-1, His Tag (Cat. No. SG1-H52H3) immobilized on CM5 Chip can bind Human CD43, His Tag (Cat. No. CD3-H52H9) with an affinity constant of 76.1 μM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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Introduction de fond

CD43 or leukosialin or sialophorin, is predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. In hematopathology, CD43 is often considered as a T-cell associated marker, because it is expressed by over 95% of thymocytes and peripheral blood T cells. CD43 positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN).

Progrès de frontière

 
Progrès du développement médicamenteux
  • Nom anglais:

    Leukosialin

  • Catégorie:

  • Nombre de médicaments répertoriés:

    0 Détails

  • Nombre de médicaments cliniques:

    0 Détails

  • Étape de développement la plus élevée:

    Preclinical

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