Human CD45 / PTPRC Protein, Llama IgG2b Fc Tag

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CD5-H5259-100ug
$410.00
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CD5-H5259-1mg (500ug X 2)
$2,575.00
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CD5-H5259
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Synonyms

CD45, PTPRC, L-CA, T200

Source

Human CD45 Protein, Llama IgG2b Fc Tag (CD5-H5259) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Lys 577 (Accession # P08575-3).

Predicted N-terminus: Gln 26

Molecular Characterization

This protein carries a llama IgG2b Fc tag at the C-terminus.

The protein has a calculated MW of 88.6 kDa. The protein migrates as 200-250 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 0.05 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 200 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Introduction de fond

CD45 is a receptor protein tyrosine phosphatase, also known as Ly-5 or leukocyte common antigen. CD45 mainly involves in the initiation of T cell receptor signaling by controlling the activation of the Src family protein-tyrosine kinases Lck and Fyn. CD45 deficiency causes in T- and B-lymphocyte dysfunction in the form of severe combined immune deficiency. It also takes a significant role in autoimmune diseases and cancer as well as in infectious diseases including fungal infections.