Human CEACAM-5 / CD66e Protein, His Tag (Mass Spec & MALS verified)

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CE5-H5226-100ug
$355.00
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CE5-H5226-1mg (500ug X 2)
$2,625.00
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Synonyms

CEACAM-5, CD66e, CEA, Meconium antigen 100

Source

Human CEACAM-5, His Tag (CE5-H5226) is expressed from human 293 cells (HEK293). It contains AA Lys 35 - Ala 685 (Accession # P06731).

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 72.2 kDa. The protein migrates as 100-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Mass Spectrometry
  • Sequence Coverage

    Human CEACAM-5, His Tag(CE5-H5226) was digested with trypsin and characterized by LC-MS/MS peptide mapping, achieving 100% sequence coverage (Routinely tested).

  • N-terminal Sequence Analysis

    Lys-Leu-Thr-Ile-Glu-Ser-Thr-Pro-Phe-Asn-Val-Ala-Glu-Gly-Lys
    (Routinely tested)

Endotoxin

Less than 0.1 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Introduction de fond

Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) is also Carcinoembryonic antigen (CEA), Meconium antigen 100, CD antigen CD66e, CEACAM5 belongs to the immunoglobulin superfamily and CEA family. CEACAM5 contains seven Ig-like (immunoglobulin-like) domains. CEACAM5 is homodimer protein which binding of E.coli Dr adhesins leads to dissociation of the homodimer. CEACAM5 is cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. CEACAM5 is receptor for E.coli Dr adhesins.

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