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Détails du produit
Synonyms
CEACAM8, CD66b, CD67, CGM6, NCA-95
Source
Biotinylated Human CEACAM-8, His,Avitag (CE8-H82E9) is expressed from human 293 cells (HEK293). It contains AA Gln 35 - Ser 319 (Accession # P31997-1).
Predicted N-terminus: Gln 35
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 35.1 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Affichage des données
SDS-PAGE
Biotinylated Human CEACAM-8, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Human CEACAM-6, His Tag (Cat. No. CE6-H5223) at 10 μg/mL (100 μL/well) can bind Biotinylated Human CEACAM-8, His,Avitag (Cat. No. CE8-H82E9) with a linear range of 0.02-0.313 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Biotinylated Human CEACAM-8, His,Avitag (Cat. No. CE8-H82E9) immobilized on SA Chip can bind Human CEACAM-6, His Tag (Cat. No. CE6-H5223) with an affinity constant of 4.57 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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FAQ
- Product
Are there any studies showing that CEACAM6 and CEACAM8 can interact?
Yes. According to the reference “Diverse oligomeric states of CEACAM IgV domains” (PNAS), CEACAMs can form homophilic and/or heterophilic interactions (e.g., CEACAM6–CEACAM8, CEACAM5–CEACAM6). Structural studies of the CEACAM6–CEACAM8 heterodimer showed that the addition of CEACAM8 to CEACAM6 led to heterodimer formation, confirming their ability to bind each other.
Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Non specific cross-reacting antigen 95
- Catégorie：
- Nombre de médicaments répertoriés：
1 Détails
- Nombre de médicaments cliniques：
1 Détails
- Étape de développement la plus élevée：
Approved
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