Mouse Contactin-4 Protein, His Tag (MALS verified)

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CO4-M52H3-100ug
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CO4-M52H3-1mg (500ug X 2)
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CO4-M52H3
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Synonyms

Contactin-4

Source

Mouse Contactin-4 Protein, His Tag (CO4-M52H3) is expressed from human 293 cells (HEK293). It contains AA Asp 19 - Ser 1000 (Accession # Q69Z26-1).

Predicted N-terminus: Asp 19

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 110.7 kDa. The protein migrates as 130-160 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background

Contactin-4 (CNTN4), a member of the contactin family of glycosylphosphatidylinositol (GPI)-anchored cell adhesion molecules, is predominantly expressed in the nervous system, especially during neural development. It mediates cell-cell interactions and axonal guidance by binding to ligands such as receptor protein tyrosine phosphatases (RPTPs) and neural cell adhesion molecules. CNTN4 plays a pivotal role in synaptogenesis, neuronal migration, and circuit formation.