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Détails du produit
Synonyms
CTLA4, CD152
Source
Cynomolgus / Rhesus macaque CTLA-4, Fc Tag (CT4-C5256) is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Ser 160 (Accession # G7PL88-1). In the region Ala 37 - Ser 160, the amino acid sequences of Cynomolgus and Rhesus macaque CTLA-4 are identical.
Predicted N-terminus: Ala 37
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 39.9 kDa. The protein migrates as 42-50 kDa under reducing (R) condition, and 80-100 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Cynomolgus / Rhesus macaque CTLA-4, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Cynomolgus / Rhesus macaque CTLA-4, Fc Tag (Cat. No. CT4-C5256) is more than 95% and the molecular weight of this protein is around 75-95 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Cynomolgus / Rhesus macaque CTLA-4, Fc Tag (Cat. No. CT4-C5256) at 5 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque B7-1, His Tag (Cat. No. CD0-C52H3) with a linear range of 0.039-1.25 μg/mL (QC tested).
Protocol
Bridging ELISA
Validated with Bispecific Antibody.
Immobilized Cynomolgus / Rhesus macaque CTLA-4, Fc Tag (Cat. No. CT4-C5256) at 1 μg/mL, add increasing concentrations of Cadonilimab and then add Biotinylated Cynomolgus PD-1 Protein, Fc, Avitagag (Cat. No. PD1-C82F7) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 0.24 ng/mL (Routinely tested).
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Cytotoxic T-Lymphocyte-Associated Antigen 4
- Catégorie：
- Nombre de médicaments répertoriés：
5 Détails
- Nombre de médicaments cliniques：
65 Détails
- Étape de développement la plus élevée：
Approved
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