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Product Details
Synonym
BLR1, CD185, MDR15
Source
Human CXCR5 Full Length Protein-VLP (CX5-H52P4) is expressed from human 293 cells (HEK293). It contains AA Asn 2 - Phe 372 (Accession # P32302-1).
Predicted N-terminus: Asp
Molecular Characterization
Virus-like particles(VLPs) are formed by self-assembly of capsid proteins from viruses. Membrane Proteins can be constituted in-situ with VLPs produced from HEK293 cell cultures. These VLPs concentrate conformationally intact membrane proteins directly on the cell surface and produce soluble, high-concentration proteins perfect for immunization and antibody screening.
The VLPs provide the display of properly folded membrane proteins in their native cellular membrane in a compact size of 100~300 nm diameter (similar to the size of most viruses) making it optimal targets for dendritic cells in vivo and surface attachment for phage display.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SEC-HPLC.
Formulation
The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 24 months under sterile conditions.
ACRO Quality Management System
- *The isotype control of empty/mock VLP (Cat. No. VLP-N5213) is sold separately and not included in protein, you can follow this link for product information.
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Performance Data
SEC-HPLC
The purity of Human CXCR5 Full Length Protein-VLP (Cat. No. CX5-H52P4) was greater than 95% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Human CXCR5 Full Length Protein-VLP (Cat. No. CX5-H52P4) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CXCR5 antibody, Human IgG4 | Human Kappa with a linear range of 1-78 ng/mL (QC tested).
Protocol
Identity-DLS
The mean peak Radius of VLP is 60-75 nm with more than 95% intensity as determined by dynamic light scattering (DLS).
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Background
Recent Advances
Drug Development Progress- English Name：
C-X-C motif chemokine receptor 5
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
2 Details
- Highest Development Stage：
Phase 2 Clinical
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