Biotinylated Human DcR3 / TNFRSF6B Protein, His,Avitag™

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Cat. No. / Size
Price
Qty
DC3-H82E3-25ug
$350.00
DC3-H82E3-200ug
$1380.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    TNFRSF6B, DCR3, TR6, M68

  • Source

    Biotinylated Human DcR3 Protein, His,Avitag (DC3-H82E3) is expressed from human 293 cells (HEK293). It contains AA Val 30 - His 300 (Accession # AAH17065).

    Predicted N-terminus: Val 30

    Request for sequence
  • Molecular Characterization

    DcR3 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 36.0 kDa. The protein migrates as 42-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    DcR3 SDS-PAGE

    Biotinylated Human DcR3 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     DcR3 ELISA

    Immobilized Human TL1A, His Tag (Cat. No. TLA-H5243) at 5 μg/mL (100 μL/well) can bind Biotinylated Human DcR3 Protein, His,Avitag (Cat. No. DC3-H82E3) with a linear range of 1-16 ng/mL (QC tested).

    Protocol
  •  DcR3 ELISA

    Immobilized Human TL1A Protein, His Tag (Cat. No. TLA-H5244) at 5 μg/mL (100 μL/well) can bind Biotinylated Human DcR3 Protein, His,Avitag (Cat. No. DC3-H82E3) with a linear range of 0.5-16 ng/mL (Routinely tested).

    Protocol
  • Bioactivity-SPR

     DcR3 SPR

    Human TL1A Protein, Fc Tag (Cat. No. TLA-H5263) captured on Protein A Chip can bind Biotinylated Human DcR3 Protein, His,Avitag (Cat. No. DC3-H82E3) with an affinity constant of 88.2 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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Background

Tumor necrosis factor receptor superfamily member 6B (TNFRSF6B) is also known as Decoy Receptor 3 (DcR3), Decoy receptor for Fas ligand and M68, which belongs to the tumor necrosis factor receptor superfamily. TNFRSF6 is a soluble protein which contains four TNFR-Cys repeats. Unlike most of the other members of TNFR superfamily, TNFRSF6 is a soluble protein which contains no transmembrane domain. TNFRSF6B acts as a decoy receptor that competes with death receptors for ligand binding. The protein is postulated to play a regulatory role in suppressing FasL- and LIGHT-mediated cell death and T cell activation as well as to induce angiogenesis via neutralization of TL1A.

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