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Biotinylated Human DDR1 Protein, His,Avitag™ (MALS verified)

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Numéro de produit/spécification
Prix
Qté
DD1-H82E9-25ug
$360.00
DD1-H82E9-200ug
$1565.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    CD167a

  • Source

    Biotinylated Human DDR1, His,Avitag (DD1-H82E9) is expressed from human 293 cells (HEK293). It contains AA Asp 21 - Thr 416 (Accession # Q08345-1).

    Predicted N-terminus: Asp 21

    Request for sequence
  • Molecular Characterization

    DDR1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 47.5 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    DDR1 SDS-PAGE

    Biotinylated Human DDR1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    DDR1 SEC-MALS

    The purity of Biotinylated Human DDR1, His,Avitag (Cat. No. DD1-H82E9) is more than 95% and the molecular weight of this protein is around 51-69 kDa verified by SEC-MALS.

    Report
  • Bioactivity-BLI

     DDR1 BLI

    Loaded Biotinylated Human DDR1 protein, His,Avitag (Cat. No. DD1-H82E9) on SA Biosensor, can bind Native Human Collagen I protein with an affinity constant of 0.603 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

    Protocol

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Introduction de fond

Discoidin domain receptor 1 (DDR1) is a member of DDRs, which are members of the receptor tyrosine kinase (RTK). Upon collagen binding, DDR1 undergoes tyrosine autophosphorylation, which consequently triggers downstream genetic and cellular pathways and plays critical roles in the regulation of cellular morphogenesis, differentiation, proliferation, adhesion, migration, and invasion. Research shows that DDR1 is closely related to various human diseases including cancer, fibrosis, atherosclerosis, and other inflammatory disorders. New generation DDR1 inhibitors targeting the allosteric sites outside of the canonical ATP-binding pocket or extracellular domain (allosteric inhibitors) may offer a new opportunity for selective DDR1 inhibition therapy development.

Progrès de frontière

 
Progrès du développement médicamenteux
  • Nom anglais:

    Discoidin domain receptor 1

  • Catégorie:

  • Nombre de médicaments répertoriés:

    0 Détails

  • Nombre de médicaments cliniques:

    5 Détails

  • Étape de développement la plus élevée:

    Phase 2 Clinical

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