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Product Details
Synonyms
Delta3, delta-like 3 (Drosophila), delta-like protein 3, DLL3, Pudgy, SCDO1, SCDO1delta3
Source
Biotinylated Human DLL3 (27-479) Protein, His,Avitag (DL3-H82Ea) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Ala 479 (Accession # Q9NYJ7-1).
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 62.2 kDa. The protein migrates as 60-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human DLL3 (27-479) Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human DLL3 (27-479) Protein, His,Avitag (Cat. No. DL3-H82Ea) is more than 85% and the molecular weight of this protein is around 65-95 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human DLL3 (27-479) Protein, His,Avitag (Cat. No. DL3-H82Ea) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-DLL3 Antibody (specific Binding EGF5 of DLL3), Human IgG1 with a linear range of 0.05-2 ng/mL (QC tested).
Protocol
Bioactivity-TR-FRET
In this TR-FRET assay, Streptavidin Protein Europium chelate (for TR-FRET) (Cat. No. FRT-TD09) is used as the Donor and Monoclonal Anti-Human IgG Antibody FA-Labeled (for TR-FRET) (Cat. No. FRT-TA02) is used as the Acceptor. Biotinylated Human DLL3 (27-479) Protein, His,Avitag (Cat. No. DL3-H82Ea), at 0.5 ng/well, can bind to the Tarlatamab with a linear detection range of 4-0.002 μg/mL (Routinely tested).
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Background
Recent Advances
Drug Development Progress- English Name：
Delta-like protein 3
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
28 Details
- Highest Development Stage：
Approved
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