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Alexa Fluor™ 488-Labeled Human EGF Protein, His TagStar Staining

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Numéro de produit/spécification
Prix
Qté
EGF-HA243-25ug
$505.00
EGF-HA243-200ug
$3295.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    EGF, URG, HOMG4

  • Source

    Alexa Fluor 488-Labeled Human EGF Protein, His Tag (EGF-HA243) is produced via conjugation of AF488 to Human EGF, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human EGF, His Tag is expressed from human 293 cells (HEK293). It contains AA Asn 971 - Arg 1023 (Accession # P01133-1).

    Request for sequence

  • Molecular Characterization

    EGF Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

  • Conjugate

    AF488

    Excitation Wavelength: 488 nm

    Emission Wavelength: 517 nm

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • Bioactivity-ELISA

     EGF ELISA

    Immobilized Alexa Fluor 488-Labeled Human EGF Protein, His Tag (Cat. No. EGF-HA243) at 5 μg/mL (100 μL/well) can bind Human EGF R, Fc Tag (Cat. No. EGR-H5252) with a linear range of 0.05-1 ng/mL (Routinely tested).

    Protocol
  • Bioactivity-FACS

     EGF FACS

    2e5 of HEK293/Human EGF R Stable Cell Line (Cat. No. CHEK-ATP148) were stained with 100 μL of 0.3 μg/mL of Alexa Fluor 488-Labeled Human EGF Protein, His Tag (Cat. No. EGF-HA243) and negative control protein respectively, washed and then analyzed with FACS (QC tested).

    Protocol

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Introduction de fond

Human epidermal growth factor (EGF) is also known as HOMG4 and URG,and is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Epidermal growth factor can be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. All family members contain one or more repeats of the conserved amino acid sequence. The biological effects of salivary EGF include healing of oral and gastroesophageal ulcers, inhibition of gastric acid secretion, stimulation of DNA synthesis as well as mucosal protection from intraluminal injurious factors such as gastric acid, bile acids, pepsin, and trypsin and to physical, chemical and bacterial agents. Because of the increased risk of cancer by EGF, inhibiting it decreases cancer risk.

Progrès de frontière

 
Progrès du développement médicamenteux
  • Nom anglais:

    Epidermal growth factor

  • Catégorie:

  • Nombre de médicaments répertoriés:

    0 Détails

  • Nombre de médicaments cliniques:

    4 Détails

  • Étape de développement la plus élevée:

    Phase 2 Clinical

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