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Human ENO1 Protein, His Tag (HPLC verified)

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Numéro de produit/spécification
Prix
Qté
EN1-H5143-100ug
$350.00
EN1-H5143-1mg
$2500.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    Enolase 1, ENO1, 2-phosphoglycerate dehydratase, ENO1L1

  • Source

    Human ENO1 Protein, His Tag (EN1-H5143) is expressed from E. coli cells. It contains AA Ser 2 - Lys 434 (Accession # P06733-1).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    ENO1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 49.1 kDa. The protein migrates as 50-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH8.0 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    ENO1 SDS-PAGE

    Human ENO1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-HPLC

    ENO1 SEC-HPLC

    The purity of Human ENO1 Protein, His Tag (Cat. No. EN1-H5143) was greater than 90% as determined by SEC-HPLC.

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Introduction de fond

Enolase 1 (ENO1), also known as alpha‑enolase, is a glycolytic enzyme that catalyzes the conversion of 2‑phosphoglycerate to phosphoenolpyruvate. It is ubiquitously expressed and accounts for the majority of cytosolic enolase activity. Beyond its metabolic role, ENO1 is a multifunctional “moonlighting” protein: on the cell surface it acts as a plasminogen receptor promoting extracellular matrix degradation and tumor invasion, while a nuclear isoform (MBP‑1) binds the c‑MYC promoter and regulates transcription. Overexpression of ENO1 has been linked to cancer progression, autoimmune diseases, and neurodegeneration, making it both a biomarker and a potential therapeutic target.

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