Enterokinase (MALS verified)

Enterokinase is expressed from Pichia Pastoris, it contained the light chain of the bovine enterokinase. Enterokinase is a specific protease that cleaves after lysine at its cleavage site Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline. This protease with 10 × His-tag binds with Ni2+ affinity chromatography and was designed for removing from digestion system.

Protein Purification.
Fusion Protein Cleavage.

1 unit is defined as the amount of enzyme required to cleave 25 µg of a MBP-EK-paramyosin-ΔSal substrate to 95% completion in 16 hours at 25°C in a total reaction volume of 100 µl.

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

>8 U/μL

Less than 1.0 EU per μg by the LAL method / rFC method.

Supplied as 0.2 μm filtered solution in 20 mM Tris, 200 mM NaCl, pH7.2 with glycerol as protectant.

Contact us for customized product form or formulation.

This product is supplied and shipped on dry ice.

• The product MUST be stored at -20°C or lower upon receipt;
• -20°C for 3 months under sterile conditions.