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Détails du produit
Synonyms
EPCAM, TACSTD1, TROP1, CD326, DIAR5, EGP2, EGP314, EGP40, ESA, GA733-2, HNPCC8, HNPCC-8, KS1, 4, KSA, M4S1, MIC18, MK1
Source
FITC-Labeled Human EpCAM, His Tag (EPM-HF225) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Lys 265 (Accession # AAH14785.1). It is the FITC labeled form of Human EpCAM, His Tag (Cat. No. EPM-H5223).
Predicted N-terminus: Gln 24
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 28.2 kDa. The protein migrates as 30-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Conjugate
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
Protein Ratio
The FITC to protein molar ratio is 1.5-3.5.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
FITC-Labeled Human EpCAM, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Anti-EpCAM Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human EpCAM, His Tag (Cat. No. EPM-HF225) with a linear range of 0.01-0.313 μg/mL (QC tested).
Protocol
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A***O2025-07-30
J'aimeI tested the FITC-EpCAM protein for binding to our anti-EpCAM CAR-T cells which were made by lentiviral transduction of human T cells . The protein stained the CAR-T cells but not the control T cells at both 10 ug/ml and 2 ug/ml. It's a good product.
Introduction de fond
Ep-CAM cross signaling with N-cadherin involves Pi3K, resulting in the abrogation of the cadherin adhesion complexes in epithelial cells was reported. And Epithelial cell adhesion molecule (Ep-CAM) recently received increased attention as a prognostic factor in breast cancer.
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Epithelial cell adhesion molecule
- Catégorie：
- Nombre de médicaments répertoriés：
1 Détails
- Nombre de médicaments cliniques：
11 Détails
- Étape de développement la plus élevée：
Approved
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