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Human EphA5 Protein, His Tag (MALS verified)

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Numéro de produit/spécification
Prix
Qté
EP5-H52H5-100ug
$360.00
EP5-H52H5-1mg (500ug X 2)
$2380.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    Ephrin type-A receptor 5

  • Source

    Human EphA5, His Tag (EP5-H52H5) is expressed from human 293 cells (HEK293). It contains AA Pro 25 - Pro 573 (Accession # P54756-1).

    Predicted N-terminus: Pro 25

    Request for sequence
  • Molecular Characterization

    EphA5 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 62.7 kDa. The protein migrates as 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    EphA5 SDS-PAGE

    Human EphA5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    EphA5 SEC-MALS

    The purity of Human EphA5, His Tag (Cat. No. EP5-H52H5) is more than 95% and the molecular weight of this protein is around 65-80 kDa verified by SEC-MALS.

    Report
  • Bioactivity-SPR

     EphA5 SPR

    Human Ephrin-A3, Fc Tag (Cat. No. EA3-H5258) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human EphA5, His Tag (Cat. No. EP5-H52H5) with an affinity constant of 0.576 μM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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Introduction de fond

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance.

Progrès de frontière

 
Progrès du développement médicamenteux
  • Nom anglais:

    EPH Receptor A5

  • Catégorie:

  • Nombre de médicaments répertoriés:

    0 Détails

  • Nombre de médicaments cliniques:

    1 Détails

  • Étape de développement la plus élevée:

    Phase 2 Clinical

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