Alexa Fluor™ 647-Labeled Human FAP Protein, His Tag

Synonyms

FAP, FAPalpha, SIMP, Seprase, APCE

Source

Alexa Fluor 647-Labeled Human FAP Protein, His Tag (FAP-HA243) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Asp 760 (Accession # Q12884-1). It is the Alexa Fluor™ 647 labeled form of Human FAP, His Tag (Cat. No. FAP-H5244).

Predicted N-terminus: His

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 86.9 kDa. The protein migrates as 90-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Conjugate

AF647

Excitation Wavelength: 640 nm

Emission Wavelength: 672 nm

Labeling

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with AF647 using standard chemical labeling method. The residual AF647 is removed by molecular sieve treatment during purification process.

Protein Ratio

The AF647 to protein molar ratio is 1-2.5.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process