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Détails du produit
Synonyms
CD32a, FCGR2A, CD32, FCG2 , FCGR2A1, IGFR2
Source
Biotinylated Human CD32a (R167), His,Avitag (CDA-H82E5) is expressed from human 293 cells (HEK293). It contains AA Ala 36 - Ile 218 (Accession # P12318-1 (H167R)).
Predicted N-terminus: Ala 36
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 23.9 kDa. The protein migrates as 30-34 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the SABA assay / HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Biotinylated Human CD32a (R167), His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human CD32a (R167), His,Avitag (Cat. No. CDA-H82E5) is more than 95% and the molecular weight of this protein is around 23-35 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human IgG1 Fc (C103S, M135Y, S137T, T139E) Protein, His Tag (Cat. No. IG1-H52H5) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD32a (R167), His,Avitag (Cat. No. CDA-H82E5) with a linear range of 0.01-1.25 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Biotinylated Human Fc gamma RIIA / CD32a (R167) Protein, His,Avitag™ (MALS & SPR verified)(Cat. No. CDA-H82E5) captured on Biotin CAP- Series S Sensor Chip can bind Rituximab biosimilar (Cat. No. CD0-M36) with an affinity constant of 0.771 μM as determined in a SPR assay (Biacore T200) (QC tested).
Protocol
Biotinylated Human CD32a (R167), His,Avitag (Cat. No. CDA-H82E5) immobilized on SA Chip can bind Rituximab with an affinity constant of 0.802 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
Introduction de fond
There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. The activating isoform, CD32A, is expressed on monocytes, neutrophils, platelets and dendritic cells. CD32A is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells), where inhibitory ITIMbearing receptors may also be coexpressed and coengaged by specific ligands. CD32A delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation and cytokine production. The responses can be modulated by signals from the coexpressed inhibitory receptors such as CD32B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Low affinity immunoglobulin gamma Fc region receptor II-a
- Catégorie：
- Nombre de médicaments répertoriés：
0 Détails
- Nombre de médicaments cliniques：
2 Détails
- Étape de développement la plus élevée：
Phase 1 Clinical
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