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Product Details
Synonyms
FCGR3A, CD16A, FCG3, FCGR3, IGFR3
Source
Biotinylated Human CD16a (V176) Protein, Avitag,His Tag (CDA-H82E9) is expressed from human 293 cells (HEK293). It contains AA Gly 17 - Gln 208 (Accession # P08637-1 (F176V)).
Predicted N-terminus: Gly 17
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 25.5 kDa. The protein migrates as 36-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Biotinylated Human CD16a (V176) Protein, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human CD16a (V176) Protein, Avitag,His Tag (Cat. No. CDA-H82E9) is more than 95% and the molecular weight of this protein is around 35-60 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Human IgG1 (S298A, E333A, K334A) Kappa Isotype Control (mAb) (Cat. No. DNP-M880) at 1 μg/mL (100 μL/well) can bind Biotinylated Human CD16a (V176) Protein, Avitag,His Tag (Cat. No. CDA-H82E9) with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
Immobilized Monoclonal Human IgG1 (S239D, I332E) Antibody Isotype Control (Cat. No. DNP-M881) at 1 μg/mL (100 μL/well) can bind Biotinylated Human CD16a (V176) Protein, Avitag,His Tag (Cat. No. CDA-H82E9) with a linear range of 0.6-78 ng/mL (Routinely tested).
Protocol
Immobilized Human IgG4 (S228P, L235E) Kappa Isotype Control (mAb) (Cat. No. DNP-M886) at 1 μg/mL (100 μL/well) can bind Biotinylated Human CD16a (V176) Protein, Avitag,His Tag (Cat. No. CDA-H82E9) with a linear range of 0.016-1 μg/mL (Routinely tested).
Protocol
Immobilized Human IgG1 Fc (C103S, D239E, L241E), Tag Free (Cat. No. IG1-H52H1) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD16a (V176) Protein, Avitag,His Tag (Cat. No. CDA-H82E9) with a linear range of 0.02-1.25 μg/mL (Routinely tested).
Protocol
Immobilized Human IgG1 Fc (C103S, M135Y, S137T, T139E) Protein, His Tag (Cat. No. IG1-H52H5) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD16a (V176), Avitag,His Tag (Cat. No. CDA-H82E9) with a linear range of 0.001-0.156 μg/mL (Routinely tested).
Protocol
Immobilized Human IgG1 Fc (C103S, N180A) Protein, His Tag (Cat. No. IG1-H52H7) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD16a (V176) Protein, Avitag,His Tag (Cat. No. CDA-H82E9) with a linear range of 0.039-1.25 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Biotinylated Human CD16a (V176), Avitag,His Tag (SPR & BLI verified) (Cat. No. CDA-H82E9) captured on Biotin CAP- Series S Sensor Chip can bind Rituximab biosimilar (Cat. No. CD0-M36) with an affinity constant of 0.367μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Immobilized Biotinylated Human CD16a (V176), Avitag,His Tag (SPR & BLI verified) (Cat. No. CDA-H82E9) on SA Chip can bind Rituximab with an affinity constant of 0.3 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human CD16a (V176), Avitag,His Tag (SPR & BLI verified) (Cat. No. CDA-H82E9) on SA Biosensor, can bind Rituximab with an affinity constant of 0.316 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
W***u2024-10-08
LikesWe used this biotin labeled protein in the ELISA assay and we have got reproducible and high-quality results. Also I want to mention and there is no degradation or activity changes of this product over more than half year, which made it our experiment easier without bridging assay.
Background
CD16a antigen is also known as Low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIII-alpha. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placentaltrophoblasts.CD16a is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibodydependent cytotoxicity and clearance of immune complexes. Aberrant expression or mutations of CD16a is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.
Recent Advances
Drug Development Progress- English Name：
Low affinity immunoglobulin gamma Fc region receptor III-A
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14 Details
- Highest Development Stage：
Phase 2 Clinical
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