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Détails du produit
Synonyms
FGFR4, CD334, JTK2, MGC20292, TKF
Source
Human FGF R4, Fc Tag (FG4-H5253) is expressed from human 293 cells (HEK293). It contains AA Leu 22 - Asp 369 (Accession # P22455-1).
Predicted N-terminus: Leu 22
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 65.0 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Human FGF R4, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human FGF R4, Fc Tag (Cat. No. FG4-H5253) is more than 90% and the molecular weight of this protein is around 145-175 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human FGF-17 Protein, His Tag (Cat. No. FG7-H5144) at 5 μg/mL (100 μL/well) can bind Human FGF R4, Fc Tag (Cat. No. FG4-H5253) with a linear range of 0.078-0.625 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human FGF R4, Fc Tag (Cat. No. FG4-H5253) immobilized on CM5 Chip can bind Human FGF-4 Protein, His Tag (Cat. No. FG4-H51H3) with an affinity constant of 1.48 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Bioactivity-BLI
Loaded Human FGF19, His Tag (Cat. No. FG9-H51H3) on HIS1K Biosensor, can bind Human FGF R4, Fc Tag (Cat. No. FG4-H5253) with an affinity constant of 16.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human FGF R4, Fc Tag (Cat. No. FG4-H5253) on Protein A Biosensor, can bind Human FGF-9, His Tag (Cat. No. FG9-H52H3) with an affinity constant of 120 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human FGF R4, Fc Tag (Cat. No. FG4-H5253) on Protein A Biosensor, can bind Mouse Klotho beta, His Tag (Cat. No. KLB-M52H7) with an affinity constant of 54.0 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Fibroblast growth factor receptor 4
- Catégorie：
- Nombre de médicaments répertoriés：
3 Détails
- Nombre de médicaments cliniques：
19 Détails
- Étape de développement la plus élevée：
Approved
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