Détails du produit
Features and Advantages
- Native Conformation: Native sequences, tag-free and natural function.
- Reliable Activity: Biological activity calibrated against WHO/NIBSC standards.
- Stringent Quality Control: Protein content, purity, and cell-based bioactivity testing for each batch.
- Lowest Endotoxin level (<0.01 EU/ug).
- Safety Assurance: Sterile filtration through 0.2 μm membrane.
- AOF: Animal origin-free raw materials throughout the production process.
Synonyms
Fibronectin, FN1, CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF
Source
Recombinant Fibronectin fragment, Research Grade (FIN-H5117) is expressed from E. coli cells. It contains AA Pro 1361 - Ser 1637 & Ala 1812 - Thr 2107 (Accession # P02751-15).
Predicted N-terminus: Met
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries no "tag".
The protein has a calculated MW of 62.6 kDa. The protein migrates as 60 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Formulation
Supplied as 0.2 μm filtered solution in 12.5 mM Citric acid, pH6.2 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C for 12 months under sterile conditions.
ACRO Quality Management System
Quality Description
ACRO's Research-grade products are suitable for a wide range of cell culture applications, particularly for research use in academic institutions. These products are sterilized by filtration, followed by lyophilization where applicable. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. Biological activity is calibrated against WHO/NIBSC standards when available.
ACRO's Premium-grade (Pre-GMP) products are characterized by their high quality and enhanced safety profiles, making them ideal for early-stage discovery and manufacturing processes in cell therapy companies. A key advantage is their seamless transition to corresponding GMP-grade versions. Biological activity is calibrated against WHO/NIBSC standards when available. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. In addition, rigorous testing is conducted to ensure the absence of mycoplasma, HCD, and HCP, thereby guaranteeing product safety.
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Affichage des données
SDS-PAGE

Recombinant Fibronectin fragment, Research Grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC

The purity of Recombinant Fibronectin fragment, Research Grade (Cat. No. FIN-H5117) was greater than 90% as determined by SEC-HPLC.
Bioactivity-SPR

Recombinant Fibronectin fragment, Research Grade (Cat. No. FIN-H5117) immobilized on CM5 Chip can bind Heparin Sulfate with an affinity constant of 0.24 μM as determined in a SPR assay (Biacore T200) (QC tested).
Protocol
Biotinylated Human ITGA5&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82Wa) immobilized on SA Chip can bind Recombinant Fibronectin fragment, Research Grade (Cat. No. FIN-H5117) with an affinity constant of 43.4 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82W1) immobilized on SA Chip can bind Recombinant Fibronectin fragment, Research Grade (Cat. No. FIN-H5117) with an affinity constant of 0.823 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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