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Human Flt-3 / Flk-2 Protein, Fc Tag (MALS verified)

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Numéro de produit/spécification
Prix
Qté
FL3-H5258-100ug
$310.00
FL3-H5258-1mg (200ug X 5)
$2245.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    Flt-3, Flk-2, STK-1, CD135, FLK2, FLT-3

  • Source

    Human Flt-3, Fc Tag (FL3-H5258) is expressed from human 293 cells (HEK293). It contains AA Asn 27 - Ser 543 (Accession # P36888-1).

    Predicted N-terminus: Asn 27

    Request for sequence
  • Molecular Characterization

    Flt-3 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 84.8 kDa. The protein migrates as 100-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    Flt-3 SDS-PAGE

    Human Flt-3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    Flt-3 SEC-MALS

    The purity of Human Flt-3, Fc Tag (Cat. No. FL3-H5258) is more than 95% and the molecular weight of this protein is around 210-275 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     Flt-3 ELISA

    Immobilized Human Flt-3 Ligand, premium grade (Cat. No. FLL-H5218) at 1 μg/mL (100 μL/well) can bind Human Flt-3, Fc Tag (Cat. No. FL3-H5258) with a linear range of 0.1-2 ng/mL (QC tested).

    Protocol

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Introduction de fond

Flt-3(Receptor-type tyrosine-protein kinase FLT3) is also known as FLK-2(Fetal liver kinase-2), STK-1(Stem cell tyrosine kinase 1), CD135. FLT3 is a cytokine receptor which belongs to the receptor tyrosine kinase class III. Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.

Progrès de frontière

 
Progrès du développement médicamenteux
  • Nom anglais:

    Tyrosine-protein kinase receptor FLT3

  • Catégorie:

  • Nombre de médicaments répertoriés:

    14 Détails

  • Nombre de médicaments cliniques:

    59 Détails

  • Étape de développement la plus élevée:

    Approved

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