Product Details
Synonyms
PGQTL2
Source
Human GIPR Protein, Fc Tag (GIR-H5253) is expressed from human 293 cells (HEK293). It contains AA Gly 26 - Gln 138 (Accession # P48546-1).
Predicted N-terminus: Gly 26
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 39.4 kDa. The protein migrates as 45-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Human GIPR Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-SPR

Human GIPR Protein, Fc Tag (Cat. No. GIR-H5253) immobilized on CM5 Chip can bind GIP, human with an affinity constant of 184 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Bioactivity-BLI

Loaded Human GIPR Protein, Fc Tag (Cat. No. GIR-H5253) on Protein A Biosensor, can bind GIP, human with an affinity constant of 64 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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